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. 2013 Nov 29;8(11):e82732. doi: 10.1371/journal.pone.0082732

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© 2013 Fecchio et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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SH-SY5Y cells were treated with aS/DHA oligomers and the number of PI-positive cells was calculated as a percentage of the total counterstained cells. Quantitation (n = 3 cultures, with 5 fields analyzed per culture, 250-300 cells per field; error bars indicated the S.E.) indicated that aS/DHA oligomers increased the percentage of PI-positive cells. Statistical significance was calculated by one-way ANOVA (p < 0.05) compared with mock control or monomeric aS.