Table 2. Main indices of genetic diversity and Hardy-Weinberg equilibrium by locus (vertical) and by population (horizontal) of the 79 Trypanosoma cruzi TcI stocks isolated from six potentially panmictic populations and of the 21 T. cruzi TcI reference strains.
Population | MCLE01 | SCLE10 | SCLE11 | MCLF10 | A427 | MCLG10 | C875 | MCLE08 | Overall | |
---|---|---|---|---|---|---|---|---|---|---|
Luribay | N/no. all/all. rich. | 6/2/1.67 | 6/3/2.58 | 6/2/1.67 | 6/2/1.91 | 6/6/4.66 | 6/2/1.67 | 6/2/1.91 | 4/1/1.00 | 2.13* |
Ho/He/F is | 0.17/0.17/0.00 | 0.50/0.44/-0.15 | 0.17/0.17/0.00 | 0.33/0.30/-0.11 | 0.50/0.82/0.41 | 0.17/0.17/0.00 | 0.00/0.30/1.00 | NA | 0.23/0.30/0.24 | |
MLG | - | - | - | - | - | - | - | - | 6 | |
Mecapaca | N/no. all/all. rich. | 11/3/1.97 | 11/2/2.00 | 11/3/2.36 | 11/2/1.92 | 11/5/2.94 | 11/1/1.00 | 11/3/2.92 | 11/2/2.00 | 2.14* |
Ho/He/F is | 0.09/0.25/0.65 | 0.73/0.52/-0.43 | 0.09/0.56/0.84 | 0.45/0.37/-0.25 | 0.18/0.47/0.63 | NA | 0.73/0.67/-0.08 | 0.36/0.52/0.31 | 0.33/0.42/0.23 | |
MLG | - | - | - | - | - | - | - | - | 10 | |
Qui-Urk | N/no. all/all. rich. | 12/3/2.13 | 12/2/2.00 | 12/2/1.83 | 12/1/1.00 | 12/1/1.00 | 12/1/1.00 | 12/1/1.00 | 12/2/1.97 | 1.49* |
Ho/He/F is | 0.17/0.30/0.46 | 0.92/0.52/-0.83 | 0.00/0.29/1.00 | NA | NA | NA | NA | 0.67/0.50/-0.33 | 0.22/0.20/-0.08 | |
MLG | - | - | - | - | - | - | - | - | 6 | |
Qui-BSIA | N/no. all/all. rich. | 4/4/4.00 | 4/2/2.00 | 4/4/4.00 | 4/1/1.00 | 4/1/1.00 | 4/2/2.00 | 4/1/1.00 | 4/1/1.00 | 2.00* |
Ho/He/F is | 0.25/0.75/0.70 | 1.00/0.57/-1.00 | 0.25/0.75/0.70 | NA | NA | 0.25/0.25/0.00 | NA | NA | 0.22/0.29/0.28 | |
MLG | - | - | - | - | - | - | - | - | 3 | |
Sap-Sap | N/no. all/all. rich. | 27/4/2.89 | 27/2/1.99 | 27/3/1.55 | 27/4/2.29 | 27/5/3.25 | 27/2/1.91 | 27/3/2.27 | 27/2/1.99 | 2.27* |
Ho/He/F is | 0.30/0.63/0.53 | 0.81/0.50/-0.64 | 0.07/0.14/0.48 | 0.74/0.54/-0.37 | 0.37/0.68/0.46 | 0.18/0.37/0.51 | 0.78/0.54/-0.44 | 0.85/0.50/-0.73 | 0.51/0.49/-0.05 | |
MLG | - | - | - | - | - | - | - | - | 24 | |
Sap-Cosi | N/no. all/all. rich. | 19/2/1.38 | 19/2/1.78 | 19/3/2.17 | 19/3/2.72 | 19/3/2.70 | 19/2/1.99 | 19/2/1.84 | 19/3/2.20 | 2.10* |
Ho/He/F is | 0.00/0.10/1.00 | 0.21/0.27/0.23 | 0.10/0.36/0.71 | 0.37/0.57/0.36 | 0.68/0.61/-0.12 | 0.31/0.50/0.38 | 0.16/0.31/0.49 | 0.84/0.52/-0.64 | 0.33/0.41/0.18 | |
MLG | - | - | - | - | - | - | - | - | 19 | |
REF | N/no. all/all. rich. | 21/15/6.07 | 21/7/4.23 | 21/6/4.26 | 21/8/4.98 | 20/10/5.31 | 21/7/3.38 | 21/13/4.60 | 21/4/3.05 | 4.49* |
Ho/He/F is | 0.43/0.91/0.54 | 0.43/0.80/0.47 | 0.14/0.80/0.82 | 0.29/0.85/0.67 | 0.25/0.87/0.72 | 0.29/0.68/0.58 | 0.29/0.74/0.62 | 0.48/0.67/0.30 | 0.32/0.79/0.60 | |
MLG | - | - | - | - | - | - | - | - | 21 | |
Overall with reference strains (N = 100) / without reference strains (N = 79) | ||||||||||
no. all | 18/7 | 7/3 | 9/7 | 8/5 | 13/8 | 7/2 | 15/4 | 4/3 | - | |
Size range** | 117-155 | 238-255 | 128-141 | 174-191 | 173-188 | 146-161 | 165-191 | 111-119 | - | |
mean all. rich. | 3.64/2.75 | 2.96/2.02 | 3.19/2.58 | 3.08/2.37 | 4.24/3.75 | 2.54/1.83 | 3.70/2.67 | 2.44/2.04 | - | |
F is | 0.56/0.57 | -0.17/-0.49 | 0.76/0.72 | 0.20/-0.07 | 0.44/0.31 | 0.48/0.41 | 0.17/-0.07 | -0.24/-0.46 | - | |
Hs | 0.46/0.38 | 0.51/0.45 | 0.45/0.39 | 0.39/0.30 | 0.51/0.44 | 0.29/0.22 | 0.38/0.31 | 0.39/0.34 | - |
N, size of the sample; no. all, number of alleles; all. rich., allelic richness; Ho, observed heterozygotes; He, expected heterozygotes; MLG, number of different multilocus genotypes in the subsample; REF, reference stocks; Hs, Nei's gene diversity; *, mean allelic richness; **Size range of alleles calculated with all stocks (including references); NA, not available because the locus is monomorphic.