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Specificity of the single-tube nested PCR for amplification of DNA from Pythium spp. Lane M 100 bp DNA ladder, lane N negative control, lane P P. insidiosum, lane P1 P. middletonii, lane P2 P. ultimum, lane P3 P. aphanidermatum, lane P4 P. deliense, lane P5 P. grandisporangium
