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Quantification of cell-associated calcium by ICP-OES. HMMs were incubated with 125 μg/ml of HA NPs for 1 h in the absence or presence of 0.125% D7 at 4 °C or 37 °C. Then, cell-associated calcium was measured by ICP-OES. Values represent the mean ± SD of n = 5. *equals p ≤ 0.01.
