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. 2013 Nov;3(11):130130. doi: 10.1098/rsob.130130

Figure 1.

Figure 1.

AROS expression in cancer and non-cancer cell lines. (a) Western blotting for the expression of AROS and SIRT1 proteins across a panel of cancer and non-cancer cell lines (see Materials and methods). β-actin is used as a loading control. (b) Quantification of Lamin AC, SIRT1 and AROS mRNA by qRT-PCR from total RNA extracted from ARPE19 non-cancer cells 72 h post-transfection. ***p < 0.001. (c) Western blotting for expression of proteins targeted by RNAi in ARPE19 cells in parallel to (b). Equal loading of protein is assessed using β-actin.