Figure 5.

Spike-timing-dependent secretion of BDNF from dendritic spines. (a) Summary of secretion of BDNF induced by paired glutamate-spike stimulation at different intervals. Numbers in parentheses represent the number of cases under each condition. (b) Critical window for the induction of potentiation and depression of glutamate responses. The percentage change in the EPSC amplitude at 20–30 min after the repetitive correlated spiking (60 pulses at 1 Hz) was plotted against the spike timing. Spike timing was defined by the time interval (Δt) between the onset of the glutamate response and the peak of the postsynaptic spike during each cycle of repetitive stimulation, as illustrated by the traces above. Only synapses with initial amplitude of less than 500 pA were included, and all responses were subthreshold for data associated with negatively correlated spiking. Calibration: 50 mV, 10 ms. (Adapted from [19].) (Online version in colour.)