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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Pharm Res. 2013 Jul 25;30(9):10.1007/s11095-013-1138-9. doi: 10.1007/s11095-013-1138-9

Fig. 2.

Fig. 2

Keap1 knockdown enhances CR-related induction of fatty acid oxidation genes and inhibition of lipogenic genes. Total RNA was isolated from livers of male C57BL/6 and Keap1-KD mice fed ad libitum (AL) or placed on 40% reduced caloric diet (CR) for 6 weeks. (a) Effect of CR on fatty acid oxidation gene expression in C57BL/6 and Keap1-KD mouse liver: Sirt1, Pparα, Cpt1a and Pgc-1α mRNA expression was quantified by RT2-PCR using SYBR green chemistry. (b) Effect of CR on Nrf2 target gene expression in C57BL/6 and Keap1-KD mouse liver. mRNA expression of Nqo1 and Gclc was quantified by RT2-PCR. (c) Effect of CR on lipogenic gene expression in C57BL/6 and Keap1-KD mouse liver: mRNA expression of Srebp1c, Lxr, Fas, Acc1, Acot-1, Fabp4 and Scd1 was quantified by RT2-PCR. All data is represented as average ± SEM (n=5) fold changes in expression over the controls. Groups without a common letter are considered significantly different from each other (p≤0.05).