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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Mol Microbiol. 2013 Oct 18;90(5):10.1111/mmi.12415. doi: 10.1111/mmi.12415

Fig. 3.

Fig. 3

Glycoprotein staining of SDS-PAGE gels of proteins from potential sugar import mutants. Mycoplasmas were grown in MB, and proteins were extracted with TX-114. Panel A, proteins found in the aqueous phase. Lanes labeled as Ma-821, -822, and -819 have transposon disruptions in MARTH_orf821, -822, and -819, respectively. The lane labeled Mar is the wild-type TnCtrl strain. Arrows refer to bands that were excised and analyzed by mass spectrometry for protein identification. Panel B, proteins that partitioned into the TX-114 phase. A protein of about 45 kDa that reacted with the glycostain is missing in the MARTH_orf 819 mutant and is indicated by an asterisk.