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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Exp Neurol. 2013 Sep 9;0:10.1016/j.expneurol.2013.09.001. doi: 10.1016/j.expneurol.2013.09.001

Figure 2. Pharmacologically elicited intracellular calcium release from RyR is significantly attenuated by PS1NTF but not PS2NTF.

Figure 2

A) Cytoplasmic expression of PS1NTF reduced the maximum amplitude (Fmax/F0) of the RyR mediated calcium transient after pharmacological stimulation of SH-SY5Y cells with 20mM caffeine (n=8), however, cytoplasmic expression of PS2NTF (n=11) has no effect when compared to untransfected control (n=8). B) AUC of calcium transients measured after pharmacological stimulation as a measure of total intracellular calcium release was significantly reduced in cells expressing PS1NTF but remained unaltered in PS2NTF expressing cells (n= 8 and 11, respectively). Data is shown as mean ± SEM with significance determined by one-way ANOVA and Bonferroni's post-hoc test. [* P <0.05; ** P <0.01]. C) Representative traces of calcium transients after pharmacological stimulation of SH-SY5Y cells expressing cytoplasmic PS1NTF or PS2NTF.