Figure 3. Isolation of viable Histone 2B:GFP^bright (GFP^bright) cells.

(A–B) FLOW cytometry histograms depicting cell count vs. GFP fluorescence intensity for CD45-/CD31-/TER119-/DAPI- cells derived from bitransgenic (BiTg) mice fed standard chow (no Dox, blue line) or BiTg mice fed doxycycline (dox) chow and treated with naphthalene (NA). Cells from dox/NA treated mice were evaluated on recovery day 6 (short term recovery, red line) or day 40 (long term recovery, green line). (C–D) Flow cytometry bivariate plots depicting the Sca1 and CD49f surface phenotype of GFP^bright (C) and GFP^dim (D) cells. (n = 7 analyses). (E) Real time RT-PCR analysis of Keratin (K) 5 and aldehyde dehydrogenase 1a1 (Aldh1a1) mRNAs in GFP^bright (small check) and GFP^dim (large check) populations. Mean ± SEM (n=4). (F) Dual immunofluorescence analysis of Keratin (K) 5 and GFP expression in cytospin preparations of GFP^bright cells. (n= 4 analyses).