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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Hepatology. 2013 Nov 20;59(1):10.1002/hep.26618. doi: 10.1002/hep.26618

Figure 2. Effects of HCV on FOXO3 posttranslational modifications.

Figure 2

A, B. HCV-infected and uninfected Huh7.5 cells were treated with 50 mM ethanol for 48h as described in the text. Nuclear (A) and cytosolic (B) extracts were analyzed by cIEF using indicated antibodies. Baseline levels of each trace were shifted as for Fig. 1. C. Summary of confirmed FOXO3 posttranslational modifications for each nuclear and cytosolic FOXO3 peak found using immunoprecipitation and PTM specific antibodies. U – ubiquitination, Me – arginine methylation, Ac – lysine acetylation, P – serine/threonine phosphorylation, pS318 – phosphorylation on Ser318, pS253 – phosphorylation on Ser253.