Table 2.

Expression of inflammatory cytokines in breast cancer.

Breast cancer	Subtype	IL-1	IL-6	TNF-α	Other CK	Technique	Reference
Carcinoma	Metastasic		6.9 pg/ml; p < 0.0001			ELISA	Zhang et al. (1999)
	No metastasic		1.1 pg/ml (serum)
	All tumors		92% (+) [69/75)			ELISA	Yamashita et al. (1994)
	All tumors		≈11 pg/ml/mg- p*
	Stage IV tumors		2859 pg/ml/mg
	Poor differentiated tumors	All components IL-1 system (+)				IHC, ELISA, RT-PCR	Singer et al. (2006)
	All tumors (50% stage II)	52–118 pg/ml (normal women 44–55 pg/ml)	72–226 pg/ml (normal women 35–77 pg/ml)	124–191 pg/ml (normal women 103–158 pg/ml)		Multiplex bead array	Lyon et al. (2008)
Cell lines
	MDA-MB-231		>1000 pg/ml		MCP-1 ≈ 500 pg/ml	Multiplex (VersaMAP Development System; R&D System Inc.)	Schmidt (2012)
					IL-8 ≈ 1000 pg/ml
					IL-2 ≈ 100–200 pg/ ml
					GM-CSF ≈ 1110 pg/ ml
					G-CSF ≈ 1000 pg/ml
	MDA-MB-435				IL-8 ≈10–20 pg/ml	Multiplex Development System; R&D System Inc.)	Schmidt (2012)
	SK-BR-3				MCP ≈ 1000 pg/ml	Multiplex (VersaMAP Development System; R&D System Inc.)	Schmidt (2012)
					IL-2 ≈ 100 pg/ml
	ZR-75-1				IL-2 ≈ 100 pg/ml	Multiplex Development System; R&D System Inc.)	Schmidt (2012)
	T47D				MCP-1 ≈100 pg/ml	Multiplex (VersaMAP Development System; R&D System Inc.)	Schmidt (2012)
					IL-2 ≈ 100–200 pg/ ml
	MCF-7				IL-2 ≈ 100–200 pg/ ml	Multiplex Development System; R&D System Inc.)	Schmidt (2012)
					IL-8 ≈ 50 pg/ml