Skip to main content
. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Hepatology. 2013 Oct 15;58(6):10.1002/hep.26593. doi: 10.1002/hep.26593

Fig. 6.

Fig. 6

CD39 deficiency exacerbates cold liver IRI and the systemic inflammatory response associated with enhanced intragraft DC maturation after orthotopic liver transplantation (CD39KO → WT B6, versus WT B6 → WT B6) with 24 hr graft preservation. (A) Serum ALT and AST levels were measured 6 hr after liver transplantation. (B) Histological assessment of liver graft injury 6 hr after liver transplantation (hematoxylin and eosin staining; ×40). (C) Liver damage was assessed by Suzuki score (congestion, vacuolization and necrosis) and necrotic areas were quantified. (D) Systemic (serum) IL-6, TNF-α, and MCP-1 levels were measured by cytokine bead array and serum IL-12p40 levels by ELISA. (E) Liver graft mDC cell surface phenotype was determined by flow cytometry, 6 hr after liver transplantation. (F), Cytokine expression by liver graft mDC, 6 hr after liver transplantation. Liver DC were gated on CD45+CD11c+B220- cells. n=5 transplants per group. *, p<0.05