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. Author manuscript; available in PMC: 2015 Mar 1.

Published in final edited form as: J Vasc Surg. 2013 Jun 12;59(3):804–813. doi: 10.1016/j.jvs.2013.03.047

Collagen Gel Contraction Assay. A. Photograph of a series of collagen gels with and without HA floating in media (dashed outline). Collagen-I alone (top row) and with HA (bottom row) seeded with WT SMCs. HA enhanced contraction of collagen resulting in smaller gel diameter at 18 hr. B. Line chart summarizes mean gel diameter ± SD at each time point for WT SMC in collagen (○), versus collagen + HA (□), and for rKO SMC in collagen (●), versus collagen + HA (■). Data represent mean ± SD from 6 independent experiments. C. Bar chart compares final gel diameter at 18 hrs. Loss of RHAMM significantly increased contraction (smaller gel diameter, *p=0.015, **p<0.01,n=6).

Collagen Gel Contraction Assay. A. Photograph of a series of collagen gels with and without HA floating in media (dashed outline). Collagen-I alone (top row) and with HA (bottom row) seeded with WT SMCs. HA enhanced contraction of collagen resulting in smaller gel diameter at 18 hr. B. Line chart summarizes mean gel diameter ± SD at each time point for WT SMC in collagen (○), versus collagen + HA (□), and for rKO SMC in collagen (●), versus collagen + HA (■). Data represent mean ± SD from 6 independent experiments. C. Bar chart compares final gel diameter at 18 hrs. Loss of RHAMM significantly increased contraction (smaller gel diameter, *p=0.015, **p<0.01,n=6).