Table 2. PCR primers used in this study.

Gene	Sequence	Amplicon length	Cycles
HIF-1α	5′-AGTGATGAAAGAATTACT-3′ (sense)	2759	35
	5′-AATAATACCACTTACAACA-3′ (antisense)
VEGF-A	5′-ATGAAGTGATCAAGTTCATGG-3′ (sense)	360	35
	5′-GGATCTTGGACAAACAAATGC-3′ (antisense)
VEGFR-1	5′-TTTCCATTTGATACTCTTAC-3′ (sense)	310	35
	5′-TCTTAGTTGCTTTACCAGGG-3′ (antisense)
MMP-2	5′-AGATCTTCTTCTTCAAGGACCGGTT-3′ (sense)	225	35
	5′-GGCTGGTCAGTGGCTTGGGGTA-3′ (antisense)
MMP-9	5′-GTTTTTGATGCTATTGCTGAGATCCA-3′ (sense)	208	35
	5′-CCCACATTTGACGTCCAGAGAAGAA-3′ (antisense)
18S	5′-AGCTAGGAATAATGGAATAG-3′ (sense)	150
	5′-AATCAAGAACGAAAGTCGGAG-3′ (antisense)

Abbreviations: HIF-1α, hypoxia-inducible factor-1α MMP-2, matrix metalloproteinase 2; MMP-9, matrix metalloproteinase 9; VEGF-A, vascular endothelial growth factor-A; VEGFR-1, vascular endothelial growth factor receptor 1.