Table 3.

Major wax esters and cholesteryl esters of human meibum

Major wax esters of human meibum
m/z	Fatty acid residues found in WE1	Fatty alcohol residues found in WE1,2	WE, % of their respective classes (unsaturated or saturated1), B	WE, % of the total WE pool1, B. Formulas for estimations: (B=A×0.82)unsat (B=A×0.18)sat	WE, % of total lipids, C (C=B×0.41)1	WE, % of total meibum, D (D=C×0.78)3	WE, % of the total WE pool (calculated using data of Nicolaides et al.2)
618.6	C18:1	i-C24:0	19.7	16.1	6.6	5.2	14.9
620.6	ai-C17:0	i/ai-C25:0	28.6	5.1	2.1	1.6	30 (the ratio of these WE was not established by Nicolaides et al.)
632.7	C18:1	i/ai-C25:0	22.4	18.4	7.5	5.8
646.7	C18:1	i-C26:0	35.3	28.9	11.8	8.6	21.5
660.7	C18:1	ai-C27:0	8.9	7.3	3.0	2.3	5.8
674.7	C18:1	i-C28:0	5	4.1	1.7	1.3	2.1
Sum of all shown WE, %				74.8	32.7	24.8	74.3

Major cholesteryl esters of human meibum
m/z	Fatty acid residues found in Chl-E2,4	Chl-E, % of the total Chl-E pool, (our data4), E	Individual fatty acid residues, % of all fatty acid residues in Chl-E2	Chl-E, % of total lipids, F (F=E×0.31)4	% of total meibum, G (G=F×0.78)3	Chl-E, % of the total Chl-E pool (calculated using data of Nicolaides et al.2)
707.7 + 681.6	C22:1 + i-C20:0	7.7	3.5 + 6.6	2.4	1.9	3.5 + 5.6 (total 10.1)
735.7 + 709.7	C24:1+ i-C22:0	11.1	7.3 + 5.3	3.4	2.7	7.3 + 5.3 (12.6)
763.7 + 737.7	C26:1+i-C24:0	13.6	3.6 + 11.7	4.2	3.3	3.6 + 11.7 (15.3)
751.7	ai-C25:0 + i-C25:0	12.7	12.5 + 1.3	3.9	3.0	12.5 + 1.3 (13.8)
791.7 + 765.7	C28:1 + i-C26:0	16.4	N/D + 13.7	5.1	4.0	N/D +13.7 (13.7)
821.8 + 793.8	C30:1 + i-C28:0	8.3	N/D + 2.2	2.6	2.0	N/D + 2.2 (2.2)
Sum of all shown Chl-E, %		69.8		21.6	16.9	67.7

¹From (Butovich et al., 2012a);

²from (Nicolaides et al., 1981);

³(McMahon et al., 2013);

⁴(Butovich, 2009a).

N/D – not detected; n/a – not available