Quantification of nerve growth factor (NGF) in (A) sciatic nerve, (B) DRG, (C) gastrocnemius, and (D) hindpaw skin. NGF levels were below the limit of detection in the spinal cord. A high-fat diet caused an increase in NGF in the DRG (A), gastrocnemius muscle (B), and hindpaw skin. While it appears that exercise decreases NGF closer to baseline levels in high-fat diet mice in the DRG and gastrocnemius, the only place where there was a significant decline in NGF content from HF-Sed to HF-Ex was in the hindpaw skin. Data are presented as means±SEM where n=6-11 per group per tissue. *=p<0.05, **=p<0.01.