sFRP3 expression in spinal cords and dorsal root ganglia at later developmental stages. A–J: Spinal cord sections from E14, E14.5, E16.5, E18.5, P0, P1, P4, P7, P15 and P30 were subjected to ISH with sFRP3 riboprobe. sFRP3 expression was detected in spinal cord dorsal horn after E14 (indicated by arrows), and persisted till P30. sFRP3 expression in the dorsal root ganglia (DRG) was up-regulated again at E16.5 (C). Arrows denote areas of sFRP3 localization in spinal cord. DRG is outlined by black dashed lines. Scale bar = 100 μm.