Figure 2. Loss of NS protein in albNS^cko livers by immunofluorescence.

(A) A significant number of albNS^cko hepatocytes still retains NS expression at postnatal day 1 (P1D). Starting from 1 and 2 weeks old (1W and 2W), most albNS^cko hepatocytes completely lose their NS expression. (B) In 3-week-old albNS^cko livers (albNS^cko-3W), strong NS signals are found in the hyperplastic ductular epithelium (CK-19⁺) and absent in the Alb⁺ hepatocytes. In 3-week-old NS^flx/flx livers (NS^flx/flx-3W), NS signals are found in scattered hepatocytes but not in the bile duct epithelium. Insets show enlarged images of the square regions. Scale bars, 25um.