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. Author manuscript; available in PMC: 2014 Jul 9.
Published in final edited form as: Biochemistry. 2013 Jun 28;52(27):10.1021/bi4004843. doi: 10.1021/bi4004843

Figure 6.

Figure 6

Effect of hydrogen peroxide on the NADPH-dependent inactivation of P450s 2E1 and 2E1 T303A. Wild-type P450 2E1 (A) and the P450 T303A mutant (B) were incubated with reductase in the absence (●) or presence (□) of 1 mM NADPH. Hydrogen peroxide was added to the reaction mixture at a concentration of 100 μM (△) in the absence of NADPH and at a concentration of 1 mM in the absence (▲) or presence (○) of 1 mM NADPH. The incubation conditions and measurement of 7-EFC O-deethylation activity are described under Experimental Procedures. The data represent the mean and standard deviations from three separate experiments.