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. Author manuscript; available in PMC: 2015 Jan 2.
Published in final edited form as: Oncogene. 2013 Mar 25;33(1):10.1038/onc.2013.96. doi: 10.1038/onc.2013.96

Figure 2.

Figure 2

A. Patterns of cell division of HEY and MDA-MB-231 PGCCs. HEY and MDA-MB-231 PGCC generated small-sized daughter cells via budding and bursting (black arrows) over a 4-day (HEY PGCC1 and MDA-MB-231) and 6-day period (HEY PGCC2) (10×). B. Nuclear morphology of HEY, SKOv3 and MDA-MB-231 PGCCs with budding daughter cells (black arrows) stained with Hoechst 33342. (a and d) Giant Multinucleated cells of HEY PGCCs and budding daughter cells (10×). (b and e) Budding daughter cells from SKOv3 PGCCs (10×). (c and f) Giant nucleated cells of MDA-MB-231 PGCCs and budding daughter cells (10×). C. Cell counts from HEY PGCCs. The total number of small-sized cells and PGCCs were counted for 15 days in a T25 flask after treatment with 450 μM CoCl2 for 72 hours. D. Slow-cycling nature of HEY PGCCs. (a–c) Single HEY PGCCs stained with PKH26 for 5 days (10×). (d–f) PKH26 fluorescence was detected in some PGCCs but not daughters in xenografts from HEY PGCCs (10×).