Table 4.

Central composite design matrix (coded) and result for the model of TEA-based esterquat synthesis.

Run no.	Enzyme amount (w/w%)	Reaction time (hour)	Reaction temperature (°C)	Molar ratio of substrates (mole)	Agitation speed (r.p.m.)	Conversion %
						Actual	Predicted
1	0	0	0	0	0	56.44	53.56
2	0	0	0	0	−1.75	47.78	43.86
3	−1	1	−1	−1	1	48.22	52.69
4	−1	−1	−1	−1	1	46.44	47.63
5	1	−1	1	−1	1	57.56	57.28
6	−1	1	1	−1	1	63.56	65.25
7	1	−1	−1	−1	−1	48.89	48.17
8	−1	1	1	−1	−1	62.89	62.84
9	1	−1	1	1	1	50.67	48.33
10	−1	1	−1	1	1	44.00	43.07
11	0	0	−1.75	0	0	39.56	41.75
12	−1	−1	−1	1	1	32.00	30.96
13	0	0	0	0	0	51.11	53.56
14	−1	−1	−1	−1	−1	44.44	45.23
15	−1	−1	1	−1	1	57.78	54.33
17	0	1.75	0	0	0	60.00	60.65
18	1	−1	−1	1	−1	25.00	25.53
19	1	−1	−1	−1	1	52.67	50.58
20	−1	−1	−1	1	−1	25.56	22.59
21	0	0	0	0	1.75	46.22	53.29
22	1	1	1	−1	−1	63.33	65.78
23	−1	1	−1	−1	−1	52.22	50.27
24	1	−1	−1	1	1	33.78	33.90
25	1.75	0	0	0	0	48.44	50.48
26	0	0	0	1.75	0	38.00	42.84
27	1	1	−1	1	−1	35.33	37.64
28	1	1	−1	−1	1	59.11	55.62
29	−1	−1	1	−1	−1	54.44	51.92
30	0	0	0	0	0	62.44	53.56
31	1	1	1	1	−1	58.00	57.94
32	0	0	0	0	0	58.00	53.56
33	0	0	0	0	0	55.33	53.56
34	−1	1	1	1	1	64.89	63.37
35	−1	−1	1	1	1	44.89	45.38
36	−1	1	−1	1	−1	34.89	34.70
37	1	1	1	1	1	67.11	66.32
38	1	1	−1	−1	−1	52.67	53.22
39	1	1	1	−1	1	71.33	68.19
40	−1.75	0	0	0	0	44.22	45.32
41	1	−1	1	−1	−1	48.89	54.87
43	0	0	0	0	0	46.89	53.56
44	0	0	0	0	0	52.00	53.56
45	−1	1	1	1	−1	54.22	55.00
46	1	−1	1	1	−1	41.33	39.96
47	−1	−1	1	1	−1	35.78	37.02
48	0	0	0	0	0	53.11	53.56
49	0	−1.75	0	0	0	38.00	40.50
50	1	1	−1	1	1	49.33	46.01