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. Author manuscript; available in PMC: 2014 Nov 15.
Published in final edited form as: J Immunol. 2013 Oct 14;191(10):10.4049/jimmunol.1300852. doi: 10.4049/jimmunol.1300852

Figure 3. Cross-reactive T cells Require the P4N for Specific Lysis of VV-A11R Peptide-Pulsed Targets.

Figure 3

(A and B). To test the necessary contact residues at P4 and P7 in vivo, an eight peak cytotoxic T lymphocyte assay was set up like previously described in Figure 1. Briefly, the 8 peak assay was split into two simultaneous four peak assays using dilutions of either CFSE vs. DDAO or CFSE vs. violet as described in the Materials and Methods. Target cell populations were injected (i.v.) into acute LCMV-infected mice (n=7) or uninfected mice (n=3). Percent specific lysis in panel F was calculated based on either the “SIY” peptide-pulsed population or the “OVA” peptide-pulsed population.