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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Immunol Res. 2013 Mar;55(0):10.1007/s12026-012-8357-8. doi: 10.1007/s12026-012-8357-8

Fig 3.

Fig 3

The C-terminal-transactivation domain of Pax5 is required for endogenous Aicda transcription in μM.2 cells. A) Schematic of the Pax5 wild-type (wt) and deletion mutants used in this study. B) Quantitative PCR of Aicda transcripts in sorted GFP+ μM.2 cells. All data were normalized to Hprt, n=3. μM.2 cells transduced with empty GFP vector was set to 1. **p<0.001, ***p<0.0001. C) Western detection of wild type and truncated Pax5 proteins in sorted GFP+ μM.2 cells. HDAC2 was detected as a loading control.