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. Author manuscript; available in PMC: 2014 Oct 17.
Published in final edited form as: Cell Rep. 2013 Sep 27;5(1):10.1016/j.celrep.2013.08.041. doi: 10.1016/j.celrep.2013.08.041

Figure 3. Depletion of BLM or GEN1 in the absence of SLX4 results in distinct chromosomal phenotypes that are dependent on MUS81 and SLX1.

Figure 3

(A) Metaphase spreads from SLX4-null cells depleted of BLM or GEN1 showing presence of segmented chromosomes (siBLM) or paired acentric fragments (siGEN1). Upper panels display whole metaphases. The boxed area is shown below at higher magnification. Paired acentric fragments (arrowhead) appear to originate from telomere-proximal fragments that affect both sister chromatids (black arrow. Chromatid breaks are also seen (red arrows). See Figure S4 for staining for telomeric DNA. (B) Examples of scoring of sectors and paired acentric fragments used for quantification. (C) Quantification of segmented chromosomes in SLX4-null and complemented cells depleted of BLM. Chromosomes with 3 or more segments were scored in the indicated cell lines. There had to be at least two paired fragments, on the p and/or q arm, extending from the centric fragment, separated by gaps or breaks, for a total of at least three segments. SLX4-null cells depleted of GEN1 showed no segmented chromosomes. (D) The acentric fragments (distinct pairs only) were quantified in SLX4-null and complemented cells depleted of GEN1. 20 metaphases were scored for each experiment shown in this figure and the analysis was blinded. Means and standard deviations are shown above each graph. Significance was determined by one-way ANOVA. p values are indicated for the statistically significant comparisons of interest.