. Author manuscript; available in PMC: 2013 Dec 1.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jan 1;878(1):10.1016/j.jchromb.2009.11.005. doi: 10.1016/j.jchromb.2009.11.005

Table 1.

LCMSMS parameters, retention times and internal standards (IStd) for buprenorphine (BUP), norbuprenorphine (NBUP), buprenorphine-glucuronide (BUP-Gluc), and norbuprenorphine-glucuronide (NBUP-Gluc).

Analyte	MRM Transition^a	DP^b (V)	EP^c (V)	CEP^d (V)	CE^e (eV)	CXP^f (V)	RT^g (min)	IStd

NBUP-Gluc	590.3>414.3	96	9.5	34	51	4	3.3	NBUP-d₃
	590.3>396.2	96	9.5	27	50	3

BUP-Gluc	644.3>468.1	101	9.5	30	45	4	4	BUP-d₄
	644.3>396.3	101	9.5	30	71	4

NBUP	414.3>340.1	86	12	26	43	4	4.2	NBUP-d₃
	414.3>326	86	12	26	40	5

NBUP-d₃	417.3>343.4	86	12	16	39	4	4.2
	417.3>326	86	12	22	43	4

BUP	468.3>396.2	86	12	18	53	6	5	BUP-d₄
	468.3>414.3	86	12	18	45	12

BUP-d₄	472.5>400.1	86	11	24	61	6	5
	472.5>415.3	86	11	24	45	8

The quantification transition is underlined

declustering potential

entrance potential

collision cell entrance potential

collision energy

collision cell exit potential

retention time.