Table 2.
Imprecision and analytical recovery for buprenorphine (BUP), and metabolites norbuprenorphine (NBUP), buprenorphine-glucuronide (BUP-Gluc), and norbuprenorphine-glucuronide (NBUP-Gluc) in plasma by LCMSMS.
| Analyte | Concentration (ng/mL) |
Total mean (n=20, ng/mL) |
Imprecision (n=20a, %RSD) | Analytical recovery (n=20a, % of target) |
||
|---|---|---|---|---|---|---|
| Pooled intra- day |
Inter- day |
Total | ||||
| BUP | 0.3 | 0.3 | 10.7 | 0 | 10.7 | 98.7 |
| 7.5 | 8.2 | 5.4 | 0 | 5.4 | 108.7 | |
| 30 | 31.6 | 6 | 0 | 5.9 | 105.2 | |
| NBUP | 1.5 | 1.5 | 11 | 6.8 | 13 | 101.3 |
| 7.5 | 7.6 | 8.5 | 0 | 8.5 | 100.7 | |
| 30 | 30.3 | 6.8 | 0 | 6.8 | 100.9 | |
| BUP- Gluc |
0.3 | 0.3 | 0 | 16.8 | 16.8 | 88.6 |
| 7.5 | 7.3 | 7.8 | 4.9 | 9.2 | 97.4 | |
| 30 | 30 | 7.2 | 4.8 | 8.6 | 100 | |
| NBUP- Gluc |
1.5 | 1.4 | 8.1 | 7.6 | 11.1 | 93.9 |
| 7.5 | 8 | 8 | 0 | 7.8 | 106 | |
| 30 | 32.5 | 6.7 | 0 | 6.7 | 108.3 | |
a
5 replicates on four different days for a total of 20.