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. 2013 Sep 4;13:34. doi: 10.1186/1471-213X-13-34

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Copyright © 2013 Lai et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Characterization of cell type in a human amniotic fluid cell (hAFC) line grown from one sample obtained via amniocentesis. (A) The hAFCs displayed morphology typical of mesenchymal cells. (B) Human amnion epithelial cells (hAEC) grown in culture for comparison. (C) Comparison of relative gene expression levels for mesenchymal and epithelial markers relative to 18S RNA; Compared with hAECs, hAFCs cells expressed high levels of mesenchymal markers, such as zinc finger E-box binding homeobox 1 (ZEB1), Twist, Vimentin and N-cadherin; conversely, hAFC did not express the epithelial marker E- cadherin. (D) Karyotype analysis showed a normal chromosomal complement (46,XX) in hAFCs grown herein. Magnification, 100x (A), 200x (B).