Table 2.
Primers used in this study
| Name | Sequence | Comments |
|---|---|---|
| attB-F |
GTCATTAATCGCCATTCAGGCTGCGCA |
To amplify 400 bp attB site in AseI of pEGFP-N1 |
| attB-R |
GTCATTAATCTCGGCCTCGACTCTAG |
|
| attL-F |
GGCGAGAAAGGAAGGGAAG |
To detect the attL site, 400 bp |
| attL-R |
ATTAACCCTCACTAAAGGGA |
|
| attR-F |
TCAAAGTAAACGACATGGTG |
To detect the attR site,520 bp |
| attR-R |
CCAACCGCTGTTTGGTCTGC |
|
| IV-R1 |
GCTTATAGATACCGTAGACAT |
TAIL-PCR to identify integration site |
| IV-R2 |
TCCCGTGCTCACCGTGACC |
|
| IV-R3 |
ATCAACTACCGCCACCTCG |
|
| IV-F2 |
CGCCACCTCTGACTTGAGCG |
|
| 5113-LF |
GTTGGGATTTGGCTCCAGCC |
Use with IV-R1 for determination of 5′ end of 5113 pseudo attP site |
| 5113-RR |
TCTTAACACCCCATTGTTCTC |
Use with IV-F2 for determination of 3′ end of 5113 pseudo attP site |
| 5156-LF |
GCCTTAATGAGGGCCAGAGC |
Use with IV-R1 for determination of 5′ end of 5156 pseudo attP site |
| 5156-RR |
GTTGACCCATTTTCCTGCTCTTCG |
Use with IV-F2 for determination of 3′ end of 5156 pseudo attP site |
| 1015-LF |
GTCCTGCCCTGACCCTTTGG |
Use with IV-R1 for determination of 5′ end of 1015 pseudo attP site |
| 1015-RR |
GCCAACCTCCAAGTTGTTGG |
Use with IV-F2 for determination of 3′ end of 1015 pseudo attP site |
| 2015-LF |
GCAAGGGGTT TTGAAACTGC |
Use with IV-R1 for determination of 5′ end of 2015 pseudo attP site |
| 2015-RR |
AAAAGTGCCTCAGAACACCAC |
Use with IV-F2 for determination of 3′ end of 2015 pseudo attP site |
| 5113-pF |
TCAAAGTAAACGACATGGTGGAGTGTAGCAGTTCCTAGGC |
ABI-REC cloning of 5113 pseudo attP site in pBCPB+ plasmid |
| 5113-pR |
ATTAACCCTCACTAAAGGGATCTTAACACCCCATTGTTCTC |
|
| 5156-pF |
TCAAAGTAAACGACATGGTGGACCTCTGCTGTGCCCAGAG |
ABI-REC cloning of 5156 pseudo attP site in pBCPB+ plasmid |
| 5156-pR |
ATTAACCCTCACTAAAGGGACCAACCACTGGCTTCCCTCG |
|
| 1015-pF |
TCAAAGTAAACGACATGGTGACCCCCACCCTGAGCTACTC |
ABI-REC cloning of 1015 pseudo attP site in pBCPB+ plasmid |
| 1015-pR |
ATTAACCCTCACTAAAGGGAACTCCCCAGCAACCAGCTTC |
|
| 2015-pF |
TCAAAGTAAACGACATGGTGATGAGACAAGAGGGACCAGG |
ABI-REC cloning of 2015 pseudo attP site in pBCPB+ plasmid |
| 2015-pR |
ATTAACCCTCACTAAAGGGAAAAAGTGCCTCAGAACACCAC |
|
| Pseudo-P1R |
CACCATGTCGTTTACTTTGA |
ABI-REC cloning of pig pseudo site |
| BCPB-S |
GCTGCAAGGCGATTAAGTTGGG |
Sequencing primer |
| EGFP-QF |
TGAACCGCATCGAGCTGAAGGG |
Real-time PCR primers, 130 bp |
| EGFP-QR |
ACCTTGATGCCGTTCTTCTGCTTG |
|
| TFRC-QF |
GAGACAGAAACTTTCGAAGC |
Real-time PCR primers, 81 bp |
| TFRC-QR | GAAGTCTGTGGTATCCAATCC |