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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Anesth Analg. 2013 Aug 19;117(4):10.1213/ANE.0b013e3182a00ae0. doi: 10.1213/ANE.0b013e3182a00ae0

Figure 5.

Figure 5

Propofol binds to the wild-type (WT) α I domain. A, The binding of propofol to the α I domain was determined using 1-aminoanthracene (1-AMA) displacement assay. Fluorescence emission spectra after the excitation of samples at 380 nm are shown. Downward arrow indicates that 1-AMA is displaced by propofol titration. The WT αL I domain contributes little to fluorescence at 520 nm. The figure is representative of 3 experiments. B, The shift of fluorescence intensity at 520 nm from 1-AMA alone + the WT α I domain alone is plotted against different concentration of propofol. Data represent mean ± SD of triplicates. Each data point represents a unique replication. Statistical analysis was performed using 1-way analysis of variance using Tukey post hoc pairwise comparisons. * P < 0.01 versus mock-treated sample (no propofol).