Figure 6. The KLF2^low phenotype of T_RM does not correlate with sustained TCR engagement.

(a) In vitro activated effector KLF2^GFP P14 splenocytes were transferred into uninfected hosts, and 12–15 days later the KLF2^GFP status of live non-vascular-associated P14 cells in spleen, blood and indicated NLTs were analyzed. N=8 from 3 independent experiments. The dotted line indicates threshold detection of KLF2^GFP expressing cells (as in Fig. 2c). (b) To evaluate TCR activation status of CD8⁺ T cells in NLT, Nur77^GFP P14 CD8⁺ T cells were transferred into C57BL/6 and either infected with LCMV or left uninfected (grey-filled). Thirty days after infection, Nur77^GFP expression in P14 CD8⁺ T cells isolated from tissue parenchyma of indicated NLTs (black line) was compared to cells from the spleen (dotted line). Black filled histograms indicate Nur77^GFP expression in P14 CD8⁺ T cells from animals injected with gp33–44 peptide 8 hours before harvest. Bar graph shows Nur77^GFP expression as mean +/− SD, normalized to spleen for each group. Data are compiled from 4 independent experiments (n=11–12). Statistical analysis is relative to spleen. (**, p<0.001; *, p<0.05; NS, p>0.05).