Table 3.

Frequencies of sera reactivity against protein spots in analyzed groups

Spot no.a	Protein	PDAC	non-PDAC	CP	AD	HS
		(N = 120)	(N = 50)	(N = 46)	(N = 12)	(N = 40)
1-4	VCL	31%	0%	9%	0%	5%
			P < 0.0001	P = 0.0024	P = 0.0193	P = 0.0005
5	PDC6I	21%	0 (0%)	7%	0%	3%
			P < 0.0001	P = 0.0361	P = 0.1221	P = 0.0033
6-9	EZR	56%	12%	2%	17%	10%
			P < 0.0001	P < 0.0001	P = 0.0135	P < 0.0001
10	hnRNPL	35%	4%	12%	0%	5%
			P < 0.0001	P = 0.0006	P = 0.0039	P < 0.0001
11	ANXA1	19%	0%	2%	0%	0%
			P = 0.0003	P = 0.0053	P = 0.2198	P = 0.0012
12	ANXA2	53%	8%	28%	0%	10%
			P < 0.0001	P = 0.0052	P = 0.0003	P < 0.0001

^aReactive protein spots numbered as shown in Additional file 1: Figure S2. Frequencies are expressed as percentage of positive sera; Fisher’s test was performed between PDAC and each control group, P-values < 0.05 were considered statistically significant.