Table 1.
Primers for RT-PCR analysis
| Gene | Primers | |
|---|---|---|
| EGFR |
Forward |
5′- GGCCTCCATGCTTTTGAGAA -3′ |
| Reverse |
5′- GACGCTATGTCCAGGCCAA -3′ |
|
| AR |
Forward |
5′-GAGTACGATAACGAACCGCACA -3′ |
| Reverse |
5′-TTTCCACTTTTGCCTCCCTTT -3′ |
|
| TNF-α |
Forward |
5′- TCCAATGGCAGAGTGGGTATG -3′ |
| Reverse |
5′- AGCTGGTTGTCTTTCAGCTTCAC -3′ |
|
| TLR4 |
Forward |
5′- GCCTTTCTCTCCTGCCTGAG -3′ |
| Reverse |
5′- AGCTCCATGCATTGGTAACTAATG -3′ |
|
| RPL4 |
Forward |
5′- GAGAAACCGTCGCCGAAT -3′ |
| Reverse | 5′- GCCCACCAGGAGCAAGTT -3′ | |
The oligonucleotide primers were designed from pig gene sequences in the GenBank NM-2140075 (for EGFR), NM-214376 (for AR), NM-214022.1 (for TNF-α), AB188301 (for TLR4), and DQ845176 (for RPL4). To avoid amplification of potentially contaminating genomic DNA, the primers were designed to span introns and intron-exon boundaries.