Table 1.
Gene | Primers | |
---|---|---|
EGFR |
Forward |
5′- GGCCTCCATGCTTTTGAGAA -3′ |
Reverse |
5′- GACGCTATGTCCAGGCCAA -3′ |
|
AR |
Forward |
5′-GAGTACGATAACGAACCGCACA -3′ |
Reverse |
5′-TTTCCACTTTTGCCTCCCTTT -3′ |
|
TNF-α |
Forward |
5′- TCCAATGGCAGAGTGGGTATG -3′ |
Reverse |
5′- AGCTGGTTGTCTTTCAGCTTCAC -3′ |
|
TLR4 |
Forward |
5′- GCCTTTCTCTCCTGCCTGAG -3′ |
Reverse |
5′- AGCTCCATGCATTGGTAACTAATG -3′ |
|
RPL4 |
Forward |
5′- GAGAAACCGTCGCCGAAT -3′ |
Reverse | 5′- GCCCACCAGGAGCAAGTT -3′ |
The oligonucleotide primers were designed from pig gene sequences in the GenBank NM-2140075 (for EGFR), NM-214376 (for AR), NM-214022.1 (for TNF-α), AB188301 (for TLR4), and DQ845176 (for RPL4). To avoid amplification of potentially contaminating genomic DNA, the primers were designed to span introns and intron-exon boundaries.