Skip to main content
. Author manuscript; available in PMC: 2014 Nov 15.
Published in final edited form as: J Mol Biol. 2013 Aug 14;425(22):10.1016/j.jmb.2013.08.007. doi: 10.1016/j.jmb.2013.08.007

Figure 2. Purity and surface plasmon resonance of soluble MART-1-specific, single-chain TCR T1 and its engineered variants.

Figure 2

T1 wt, T1-S18, and T1-S18.45 were expressed in the E.coli pET28 expression system, refolded from inclusion bodies, and purified by Ni-column and size exclusion chromatography. (a) SDS-PAGE of purified scTvs and molecular weight markers (M). (b) SPR trace of MART-1 (ELAGIGILTV)/HLA-A2 binding immobilized T1-S18.45. Fitted parameters (KD, kon, koff) are shown in the inset. (c) Table showing the binding affinities of the T1-derived TCRs for variants of the MART-1 peptide.