FIG. 8.

Diagram showing the parallel-plate flow chamber for vertical-step flow. The polycarbonate base plate (top), two gaskets with different open areas, and the glass slide with EC monolayer (bottom) are held together by a vacuum suction applied at the perimeter of the slide, forming a channel with a lesser depth at the entrance, creating a step. Cultured medium enters at inlet port through entrance slit into the channel and exits through exit slit and outlet port.