Table 1.
FACS enriches for H. pylori cell shape mutants from mixed cultures of mutant and wild-type
| Starting ratio ∼ 1:1 mutant : WT | Starting ratio ∼ 1:1000 mutant : WT | ||||||
|---|---|---|---|---|---|---|---|
| Strains | Shapes | Starting ratio (mut : WT) | Sorted population (mut : WT)a | Fold enrichmentb | Starting ratio (mut : WT) | Sorted population (mut : WT) | Fold enrichmenta |
| WT + Δcsd4 |
Helical + straight |
0.97 | 131.00 | 135.1 | 0.00097 | 0.15 | 154.6 |
| WT + ΔccmA |
Helical + curved |
2.30 | 510.00 | 221.7 | 0.00230 | 0.03 | 13.0 |
| WT + Δcsd3 |
Helical + var/‘c’ |
0.30 | 9.90 | 33.0 | 0.00030 | 0.11 | 366.7 |
a
Underlined values indicate only mutant was recovered from these populations; wild-type valued as our lowest level of detection for ratio calculation. Mixtures of Δcsd4, ΔccmA or Δcsd3 and wild-type were sorted using gates 1, 2 and 3, respectively, as depicted in Fig. S1. Strains used: NSH57, LSH18, KGH10, LSH112.
b
Fold enrichment is calculated as the sorted ratio divided by the starting ratio.