Figure 10.

Sequence conservation of G-quadruplex B′ motif that overlaps alternatively spliced 5′ splice site of MECP2 pre-mRNA. Location-conserved G-quadruplex B′ (which partially overlaps with G-quadruplex B) is also highly conserved at the sequence level in all the four mammalian MECP2 orthologs. Additionally, the B′ G-quadruplex was found to overlap the second 5′ splice site of MECP2 pre-mRNA. This particular site is known to be alternatively spliced in human and mouse MECP2 orthologs. The guanine groups which form the G-tetrads are underlined. G-quadruplex B′ refers to the corresponding marked map position in Figure 8.