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A, B, Immunofluorescence of NG2 revealed with Alexa Fluor 546-conjugated anti-rabbit IgG in cuprizone-treated mice (A) and cuprizone+T3-treated mice (B) at w12 + 3. C, D, Double labeling of Olig2 immunoperoxidase detected with VIP in cell nuclei (dark color) and CA II immunofluorescence in corpus callosum of cuprizone+T3-treated mouse (C, D). CA II+ cells and Olig2+ cell nuclei (arrows) are predominantly present in demyelinated area. Magnification: A–C, ×400; D, ×900.
