Figure 4.

VGLUT1 terminals in the rat are presynaptic to PKCγ interneurons. A, B, VGLUT1-immunoreactive axon terminals form synapses (arrowheads) onto the dendrites of some PKCγ neurons in the dorsal horn of spinal cord segments C6 (A) and L3 (B). VGLUT1 immunoreactivity is denoted by the amorphous electron-dense peroxidase reaction product and PKCγ immunoreactivity by the presence of TMB-tungstate crystals (stars). Unlabeled dendrites (d), as well as a PKCγ-immunoreactive dendrite, receive synapses (arrowheads) from each VGLUT1 terminal. Scale bars, 500 nm.