Figure 4.
Motor neuron survival in the presence of astrocytes. Motor neuron survival 48 h after plating either on nontransgenic or SOD1G93A astrocyte monolayers as indicated. The non-Tg astrocyte monolayers were previously treated with rotenone (Rot; 2 μm), antimycin A (AA; 5 μm), sodium azide (Az; 5 μm) or fluorocitrate (FC; 100 μm) and the SOD1G93A astrocyte monolayers with l-NAME (5 mm), DMPO (75 mm), or MnTE (10 μm) for 24 h as indicated and washed, and then a nontransgenic motor neuron suspension was added. Motor neuron counting was performed as described in Materials and Methods. Data are presented as a percentage of the non-Tg control group. Data are mean ± SEM from three independent experiments. *p < 0.05, significantly different from non-Tg control.