Table 3.

Quantification of proportional length of neocortical domains in the frontoorbital/motor area in Pax6cKO and control mice at P10

Genotypes	Id2(−)			Cdh6(−)			EphA7(+)			Mean of all markers
	NCX	Domain	Ratio	NCX	Domain	Ratio	NCX	Domain	Ratio	NCX	Domain	Ratio
WT n	4	4	4	5	5	5	6	6	6	15	15	15
Mean	17.53	6.48	0.37	17.22	5.2	0.3	17.38	5.67	0.33	17.38	5.69	0.33
± SD	0.21	0.83	0.04	0.25	1.12	0.06	0.2	0.52	0.03	0.15	0.79	0.04
Pax6cKO n	5	4	4	5	5	5	6	6	6	16	15	15
Mean	13.6	2.38	0.14	13	1.5	0.11	12.67	2.13	0.17	13.1	1.95	0.15
± SD	0.42	0.75	0.09	0.5	0.5	0.03	0.52	0.29	0.02	0.37	0.36	0.02
p <	0.0001	0.0005	0.0005	0.0001	0.0001	0.0001	0.0001	0.0001	0.0001	0.0001	0.0001	0.0001

Proportional quantification of distinct cortical domains based on the regional expression of cortical areal markers in the Pax6cKO and control brains. The title of the table indicates the analyzed cortical domain and the regional markers used in the performed radioactive in situ hybridization assays (see also supplemental Fig. 1, available at www.jneurosci.org as supplemental material). The ratio between the length of each expression domain of the markers used and the length of the NCX in the corresponding sections was calculated individually for the three levels of sectioning (levels 3–5 indicated in the schema in Fig. 3) of control and Pax6cKO brains. The last column contains the averaged values of the estimated ratios for the markers used. The p values relative to observed differences are listed below each column. Results are synthesized in histograms in Figure 3F–I.

*Not statistically proven differences (p > 0.05) between the compared groups by two-tailed, unpaired Student's t test.