Figure 4.

Lobula neuron morphology. A, B, Reconstructed layer 1–4 neurons filled with fluorescent dye. Note that the neurons project to the rim of the lobula and have highly regular projections. C, D, Reconstructed layer 5–6 neurons. These neurons do not project to the rim of the lobula and have less regular branching patterns. E, An example of the six recorded and filled columnar neurons. F, Multiple neurons filled in the same brain. The blue- and green-labeled neurons project to layers 5 and 6, whereas the red-labeled neuron projects to layers 3 and 4. G, H, Some of these neurons were reconstructed in three dimensions to examine their branching pattern relationships to the layers. Note how the layer 1–4 neuron (red) forms a rim around the layer 5–6 neuron (blue). A–F, The lobula is outlined with white dotted line. Scale bars, 100 μm. Abbreviations are as defined in Figure 2 and Results.