Figure 2.
MicroRNA levels are decreased in Dicer mutant mice. A, Northern blot analysis for mir-132 at P15 demonstrates a decrease in miRNA levels in the cortex and hippocampus of Dicerflox/flox; CaMKII Cre mice. B, qRT-PCR analysis of mir-132 levels in the cortex and hippocampus of Dicer mutant mice at P21 demonstrates that miRNA levels continue to decrease over time. U6 was used as a loading control in all experiments. Five micrograms of total RNA were loaded per well for cortical and hippocampal mir-132 Northern blot analysis. Twenty nanograms of total RNA were used to generate cDNA for qRT-PCR analysis. Error bars represent the SEM. Values are statistically significant using a one-way ANOVA (*p < 0.001) with a Tukey's multiple comparison-post test.