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Quantitative real-time PCR analysis to identify mRNAs for P2X receptor subunits and their relative expression levels. Samples of mRNA were prepared from total mouse cortex (a) or from FACS-sorted cortical astrocytes (b) of postnatal mice (P6) and were subjected to qRT- PCR. Data represent mRNA levels relative to ATP5B and are given in means ± SEM.
