Figure 7.

The CB₂ agonist JWH-133 reduces IFN-γ-inducible iNOS and CCR2 mRNA expression in microglial cells. Relative expression levels of CB₂, iNOS, and CCR2 mRNA were determined by quantitative real-time PCR. BV-2 microglial cells were stimulated either with 50 U/ml IFN-γ for 15 h alone, or in the presence of JWH-133 (5 μm) with or without CB₂ receptor antagonist SR144528 (1 μm). cDNA was generated from BV-2 cells and expression of CB₂, iNOS, and CCR2 mRNA was evaluated by real-time RT-PCR. Quantitative RT-PCR results are expressed as a ratio of average copies per copy of GAPDH for mean values ± SEM of n = 6 for all conditions. *p < 0,05; **p < 0,005 (BV-2 cells stimulated with IFN-γ vs with IFN-γ in the presence of 5 μm JWH-133).