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Ratio of 5th and 3rd harmonics of two populations of mNPs, conjugated with 15 mer and 29 mer anti-thrombin aptamer respectively, determined by MSB measurement as a function of thrombin concentration. Three control samples are applied in this experiment to verify the specificity of the detection: mNP without aptamer functionalized, and only one population of mNP functionalized with either 15 mer or 29 mer anti-thrombin aptamer with the increasing concentration of thrombin added.
