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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Nat Methods. 2013 Sep 29;10(11):1116–1121. doi: 10.1038/nmeth.2681

Fig. 3.

Fig. 3

Orthogonal recognition of crRNAs in E. coli. Cells with all 16 combinations of Cas9 and crRNA were challenged with a plasmid bearing a matched or mismatched protospacer and appropriate PAM. Sufficient cells were plated to reliably obtain colonies from matching spacer and protospacer pairings. Total colony counts on the resulting 32 plates were used to calculate the fold depletion. Values less than one were set to one for clarity.