Table 1.
Protospacer-adjacent motifs for each Cas9. We defined two thresholds of activity for PAMs. The moderate threshold required >100-fold depletion of sequences matching the protospacer as well as >50-fold depletion of all sequences with one additional base defined (plain text). The stringent PAM threshold required >500-fold depletion of sequences matching the protospacer as well as >200-fold depletion of all sequences with one additional base defined (bold).
| NM | ST1 | TD |
|---|---|---|
|
| ||
| NNNNGANN | NNAGAA | NAAAAN |
| NNNNGTTN | NNAGGA | NAAANC |
| NNNNGNNT | NNGGAA | NANAAC |
| NNNNGTNN | NNANAA | NNAAAC |
| NNNNGNTN | NNGGGA | |