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. 2013 Oct 24;109(11):2842–2852. doi: 10.1038/bjc.2013.670

Figure 3.

Figure 3

ING4-regulated cell growth and tube formation of HUVECs is partially ILK dependent. (A) Real-time PCR analysis of the expression levels of ING4 and ILK in ING4 overexpression or KD MMRU cells. *P<0.05; ***P<0.001, compared with the vector control group. (B) Real-time PCR analysis of the expression levels of ILK and ING4 in MMRU cells with ILK overexpression or KD. ***P<0.001, compared with the vector control group. The CM were collected from MMRU and MMLH cells with ING4 overexpression, ILK overexpression, ING4/ILK co-overexpression for detection of HUVECs growth by SRB assay (C) and the tube formation of HUVECs (D) (scale bar 40 μm). The number of tubes was counted in five random fields for each group. **P<0.01; ***P<0.001, compared with vector control group. ###P<0.001, compared with ING4 overexpression group. Student's t-test.