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. 2013 Oct 24;109(11):2842–2852. doi: 10.1038/bjc.2013.670

Figure 5.

Figure 5

ING4 regulates ILK/NF-κB/VEGF signalling pathway via JWA. (A) The protein levels of JWA, ING4, ILK and VEGF in MMRU cells with ING4 overexpression, or JWA KD, or ING4 overexpression/JWA KD were detected by western blot. (B) Real-time PCR analysis of the mRNA expression levels of ING4, JWA, ILK, IKK, IL-6 and VEGF in MMRU cells with ING4 overexpression, or JWA KD, or ING4 overexpression/JWA KD. *P<0.05; **P<0.01; ***P<0.001, compared with vector control group. ##P<0.01; ###P<0.001, compared with ING4 overexpression group. (C) The effect of JWA on ING4-regulated p-p65 and total p65. After transfection 48 h, the change of p-p65 and the nucleus translocation of p65 in MMRU cells with ING4 overexpression, JWA KD, or ING4 overexpression/JWA KD were checked by immunofluorescence by confocal microscopy (scale bar 10 μm).